Cloning and Sequencing Analysis of Glycerol - 3 - phosphate Acyltransferase Gene of Brassica oleracea L. var. acephalea DC.
MA Hui，LI Li-li，ZHU Hong-yan，CHEN Li-jing，ZHONG Ming，GUO Zhi-fu
College of Biological Science and Technology， Shenyang Agricultural University， Key Laboratory of Agricultural Biotechnology of Liaoning Province， Shenyang 110161， Liaoning， China
Abstract：With Brassica oleracea L. var. acephalea DC. variety‘Baikongque’as experimental material，by homology cloning and RACE method，we isolated from low temperature induction of Brassica oleracea L. var. acephalea DC. seedlings the full gene sequences of Brassica oleracea L. var.acephalea DC. glycerin-3-phosphate acyltransferase（GPAT） gene，and analyzed the sequences for bioinformatics by biology software．The results show that this gene is 1 558 bp in length，speculated encoding 451 amino acids，and is named BaGPAT．The BaGPAT gene sequence has 54%-85% similarities in amino acids，when compared with other species in sequencing analysis．The results of clustering analysis show that the genetic relationship of BaGPAT，Arabidopsis thaliana GPAT and Lepidium latifolium GPAT is closer.
