Abstract：Purple corm flesh fiber in taro is warmly welcomed by the market owing to its potential healthcare function，which is considered to be an important quality trait．This experiment took‘Lipuyu’（color of corm flesh fiber-purple）as female parent and‘Lepingyeyu’（color of corm flesh fiber-yellow）as male parent，constructed F2 segregation population and utilized specific length amplified fragment sequencing （SLAF-seq），combining bulked segregant analysis（BSA），and developed molecular markers linked to the color of corm flesh fiber in taro．The results indicated that a total of 37.65 Mb effective reads were obtained through SLAF-seq，293 363 high quality SLAFs were developed and 57 710 SNPs were obtained．And 27 SNP locus closely linked to the color of corm flesh fiber in taro were identified according to association analysis of the SNP-index．AS-PCR （allele specific PCR） primers were designed to validate the polymorphism of SNP locus．Among the 27 markers，Marker44764 was effective in genotyping of the plants with target traits．A simple and quick method for taro genotyping was established in this study，which can be utilized in efficient molecular marker-assisted selection of materials at the seedling stage，thus improving the efficiency of taro breeding．
孙亚林 王直新 季 群 刘玉平 黄新芳 朱红莲 柯卫东. 基于SLAF-seq 技术开发芋肉纤维颜色性状的分子标记[J]. 中国蔬菜, 2021, 1(6): 49-54.
SUN Yalin，WANG Zhixin，JI Qun，LIU Yuping，HUANG Xinfang，ZHU Honglian，KE Weidong*. Development of Molecular Marker for Color of Corm Flesh Fiber in Taro Based on Using SLAF-seq Technology. China Vegetables, 2021, 1(6): 49-54.
