Studies on Rapid Identification on Purity of Chinese Cabbage‘Niupai No.19’Hybrid Seed
LIU Shuan-tao，ZHANG Zhi-gang，WANG Li-hua，WANG Rong-hua，LI Qiao-yun，ZHAO Zhi-zhong*
Vegetable and Flower Institute of Shandong Academy of Agricultural Sciences，Shandong Branch of National Center for Vegetables Improvement，Shandong Key Laboratory for Biology of Greenhouse Vegetables，Huang-Huai-Hai Region Scientific Observation and Experimental Station of Vegetables，Ministry of Agriculture and Rural Affairs，Ji’nan 250100，Shandong，China
Abstract：Taking Chinese cabbage‘Niupai No.19’hybrid seeds as test material，this paper constructed a rapid molecular marker testing technique system used before Chinese cabbage hybrid seeds being stored in genebank by every link，including seed pr-treating，DNA easy isolation and quality detection，primers screening，etc.and compared it with the result from fields identification．The results showed the identification results by molecular marker from the system were basically consistent with that by field planting．The purity of hybrids was about 97%．Taking the single plant seedlings treated for 72 hours to rapidly etract DNA by 2-step DNA isolation were sufficient for completely satisfying the needs of molecular marker detection．
刘栓桃，张志刚，王立华，王荣花，李巧云，赵智中. 牛牌19 大白菜杂交种种子纯度快速鉴定方法研究[J]. 中国蔬菜, 2020, 1(3): 28-32.
LIU Shuan-tao，ZHANG Zhi-gang，WANG Li-hua，WANG Rong-hua，LI Qiao-yun，ZHAO Zhi-zhong. Studies on Rapid Identification on Purity of Chinese Cabbage‘Niupai No.19’Hybrid Seed. China Vegetables, 2020, 1(3): 28-32.
